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DNA ligase one: ligates the nascent DNA with the lagging strand once the Ribonuclease H has taken out the RNA primer from the Okazaki fragments.
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DNA ligases have grown to be indispensable applications in fashionable molecular biology exploration for producing recombinant DNA sequences. Such as, DNA ligases are made use of with restriction enzymes to insert DNA fragments, typically genes, into plasmids.
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The ultimate extract contained ten% with the action in the beginning recorded during the E. coli media; together the procedure it had been found out that ATP and Mg++ were being important to improve the response. The widespread commercially offered DNA ligases have been at first found in bacteriophage T4, E. coli and various bacteria.[17] Problems[edit]
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